Biochem/physiol Actions

Primary Targetprotein biosynthesis

General description

Potency: ≥750 µg/mg

Contains more than 98% kanamycin A. An aminoglycoside antibiotic effective against Gram-positive and Gram-negative organisms. Inhibitor of protein biosynthesis that acts on the 30S ribosome, causing misreading of the genetic code. May cause renal damage and ototoxicity.

Contains more than 98% kanamycin A. An aminoglycoside antibiotic affective against gram-positive and gram-negative organisms. Inhibitor of protein biosynthesis that acts on the 70S ribosome, causing misreading of the genetic code. May cause renal damage and ototoxicity.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Lech, K., and Brent, R. 1995. in Current Protocols in Mol. Biol. Vol. 1 (Ausubel, F.M., et al., eds.) John Wiley & Sons, New York.Kahn, R.W., et al. 1993. Proc. Natl. Acad. Sci. USA90, 9295.Cardon, G.H., et al. 1993. Plant J.3, 773.Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, New York. p.1.6, A.6.Korzybyski, T., et al. 1978. In Antibiotics, Origin, Nature, and Properties1, 690.Stiffler, P.W., et al. 1974. Antimicrob. Agents Chemother.6, 516.Umezawa, S., et al. 1968. J. Antibiot.21, 367.

Packaging

5, 25 g in Plastic ampoule

Reconstitution

Following reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.